Topics
A to Z
Data &
 Statistics
Forms &
Publications
News &
Advisories
Licensing &
Certification
Rules &
Regulations
Public Health
Directory

Enteric Pathogen Culture, Bacterial

Updated

12/2/2016

Other Names Stool Culture, Bacterial; Salmonella Culture; Shigella Culture; Escherichia coli O157 Culture; STEC Culture; Campylobacter Culture
How to Order
Check the appropriate box under the CULTURE section of the General Microbiology Request Form (form 60)
Availability
All clients for public health investigations
Methodology
Standard reference procedures for culture of Salmonella, Shigella, Campylobacter and Shiga toxigenic Escherichia coli; submitted samples can also be tested for Vibrio, Yersinia, Aeromonas, and/or Plesiomonas  upon request; any individual organism cultured upon request
Performed
Daily, on regular business days
Time to Reporting
3 to 8 working days after receipt of specimen
CPT Code
87045 – aerobic stool culture, with isolation and preliminary examination, Salmonella and Shigella  species
 
87046 – additional pathogens, isolation and presumptive identification of isolates
 
87077 – additional methods required for definitive identification, each isolate  


Specimen Collection Details

Collection
Stool in Enteric Pathogen Transport (EPT) media (e.g. Cary Blair).
 
Rectal swab in EPT media acceptable for specimens from infant patients.
Optimal Volume
5 gm solid stool or 5 ml liquid stool
Minimum Volume
2 gm solid stool or 5 ml liquid stool
Handling
Place in biohazard bag; Store specimens at refrigerated temperatures pending transport.  Do not freeze, avoid extreme heat.
Transport
Transport at refrigerated temperatures. Specimens should be received at the OSPHL within 24 hours of specimen collection.
Rejection Criteria

Rectal swabs with no visible fecal material, specimens mixed with urine, or specimens received 7 or more days from specimen collection.

Specimens are subject to the requirements of the OSPHL Specimen Submission Policy and Criteria, available at: http://bit.ly/SpecimenCriteria.

Comments
For additional information or assistance, please contact the OSPHL at 503-693-4100.