Topics
A to Z
Data &
 Statistics
Forms &
Publications
News &
Advisories
Licensing &
Certification
Rules &
Regulations
Public Health
Directory
Print this Article   Bookmark and Share

Hepatitis C Antibody, IgG & IgM

Updated

10/5/2009

Other Names Anti-HCV; HCV Ab; Anti-HCV screen
How to Order
Check the Hepatitis C Antibody – Screen Only box on the Virology/Immunology Request Form (form 42) and complete the reason for test under Billing Information Only
Availability
All clients
Methodology
Enzyme Immunoassay (EIA)
Performed
Monday, Wednesday, Friday
Time to Reporting
1 to 3 working days after receipt of specimen
CPT Code
86803


Specimen Collection Details

Collection
One 7 ml red top tube or serum separator tube (SST). Also acceptable are specimens collected with anticoagulants such as EDTA, sodium citrate or heparin. Samples collected using anticoagulant should be filled as per manufacturer’s instructions to avoid improper dilution. 
Optimal Volume
Blood: 5 ml; Serum 1.5 ml
Minimum Volume
Blood: 3 ml; Serum 0.5 ml
Handling
After blood clots, keep specimen refrigerated until transport. DO NOT FREEZE.  If possible remove the serum from the clot as soon as possible. Sample must be tested within 7 days of collection.   
Transport
Refrigerated at 2-8° C. 
Rejection Criteria
Specimens contaminated with bacteria or that have been heat-treated.
Comments
This test is intended for the detection of antibody to hepatitis C virus in human serum or plasma. Hepatitis C virus is now known to be the causative agent for most, if not all, blood-borne non A and non B hepatitis. Anti-HCV screening test results with signal/cutoff ratios greater than or equal to 3.8 usually (about 95% of the time) confirm positive. Fewer than 5 of every 100 samples with these results are usually false-positive.