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Escherichia coli O157 / Identification

Updated

4/12/2010

Other Names Escherichia coli O157 confirmation; EHEC; Enterohemorrhagic Escherichia coli; Shiga toxigenic Escherichia coli; Verotoxigenic Escherichia coli; Hemolytic Uremic Syndrome (HUS)
How to Order
Check the appropriate box, under ISOLATE IDENTIFICATION (R/O OR CONFIRM), on the General Microbiology Request Form (form 60)
Availability
All clients
Methodology
Non-sorbitol fermenting isolates are tested for O:157 and H:7 antigens. Standard reference procedures are used for the biochemical identification of Escherichia coli.  All O:157 Escherichia coli are tested for Shiga toxin 1 and Shiga toxin 2 by PCR. 
Performed
Daily, on regular business days; PCR testing is batched
Time to Reporting
4 to 15 working days
CPT Code
87077 - aerobic culture definitive identification

 

87147 - immunologic grouping, per antiserum

87801 - amplified probe, PCR

 



Specimen Collection Details

Collection
Actively growing isolated organism, on an agar slant or plate media.
Optimal Volume
N/A
Minimum Volume
N/A
Handling
Seal plate or tube, place in biohazard bag; keep at ambient or refrigerator temperature.  Do not freeze, avoid extreme heat. Submit specimen according to Infectious Substance Shipping Guidelines.
Transport
Room temperature or wet ice
Rejection Criteria
Unlabeled or leaking specimens; incomplete requisition
Comments
E. coli O157 isolates are subjected to PFGE (pulse field gel electrophoresis).  For addition information or assistance, please contact the laboratory at  503.693.4100