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Escherichia coli O157 / Identification | | Updated | 4/12/2010 | | Other Names | Escherichia coli O157 confirmation; EHEC; Enterohemorrhagic Escherichia coli; STEC; Shiga toxigenic Escherichia coli; Verotoxigenic Escherichia coli; Hemolytic Uremic Syndrome (HUS) | | How to Order | Check the appropriate box, under ISOLATE IDENTIFICATION (R/O OR CONFIRM), on the General Microbiology Request Form (form 60) | | Availability | All clients | | Methodology | Non-sorbitol fermenting isolates are tested for O:157 and H:7 antigens. Standard reference procedures are used for the biochemical identification of Escherichia coli. All O:157 Escherichia coli are tested for Shiga toxin 1 and Shiga toxin 2 by PCR. | | Performed | Daily, on regular business days; PCR testing is batched | |
Time to Reporting
| 4 to 15 working days | |
CPT Code
| 87077 - aerobic culture definitive identification
87147 - immunologic grouping, per antiserum
87801 - amplified probe, PCR
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Specimen Collection Details
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Collection
| Actively growing isolated organism, on an agar slant or plate media. | |
Optimal Volume
| N/A | |
Minimum Volume
| N/A | |
Handling
| Seal plate or tube, place in biohazard bag; keep at ambient or refrigerator temperature. Do not freeze, avoid extreme heat. Submit specimen according to Infectious Substance Shipping Guidelines. | |
Transport
| Room temperature or wet ice | |
Rejection Criteria
| Unlabeled or leaking specimens; incomplete requisition | |
Comments
| E. coli O157 isolates are subjected to PFGE (pulse field gel electrophoresis). For addition information or assistance, please contact the laboratory at 503.693.4100 |
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