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Rubella Antibody, IgG

Updated

8/15/2013

Other Names Rubella IgG, German measles (Rubella), 3 day measles 
How to Order
Check the Rubella IgG box on the green Virology form 42
Availability
All clients
Methodology
Enzyme Immunoassay (EIA)
Performed
Tuesday and Thursday
Time to Reporting
24 - 72 hours after receipt of specimen at OSPHL
CPT Code
86762


Specimen Collection Details

Collection
One 7 ml red top tube or serum separator tube (SST).
Optimal Volume
Blood: 5-7 ml; Serum 1-2 ml
Minimum Volume
Blood: 1 ml; Serum 0.5 ml
Handling
Blood: After blood clots, keep specimen refrigerated until transport. Samples that will be tested within 3 days of collection can be stored on the clot. If possible remove the serum from the clot as soon as possible. DO NOT FREEZE. Sample must be tested within 7 days of collection.
Transport
Transport refrigerated at 2-8° C within 5 days.
Rejection Criteria
Specimens that have undergone multiple freeze/thaw cycles, are Hemolyzed, Icteric, heat inactivated or grossly contaminated, are unacceptable for testing.
Comments
The level of 10 IU/mL of rubella antibody is accepted as the cutoff for immunity.

 

 

A POSITIVE result corresponds to a level > 10 IU/mL. Specimen is positive for anti-Rubella IgG and the patient is presumed to be immune.

 

A Negative result corresponds to an antibody level <9 IU/mL. Speciemn is negative for anti-Rubella IgG and the patient is presumed to be non-immune.

 

An Equivocal result corresponds to an antibody level between > 9 but < 10 IU/mL. The specimen is Equivocal for anti-Rubella IgG antibody and the patient’s immune status cannot be determined. A subsequent sample should be drawn and tested simultaneously with the initial sample. If the subsequent sample is positive, seroconversion has occurred and may be indicative of recent infection. If the subsequent sample also tests as Equivocal then antibody status is undetermined and the sample is deemed equivocal.

 

This test is used for the qualitative and/or semi-quantitative detection of IgG antibodies to rubella in human serum. The Rubella IgG ELISA test is of value in the determination of rubella immune status. Seroconversion between acute and convalescent sera is considered strong evidence of current or recent infection. Note: the Acute phase serum specimen should be drawn as soon after rash onset as possible, within the first 7 days. The Convalescent phase serum should be drawn 10 days or more after the Acute phase specimen.