Topics
A to Z
Data &
 Statistics
Forms &
Publications
News &
Advisories
Licensing &
Certification
Rules &
Regulations
Public Health
Directory

Hepatitis B Surface Antigen

Updated

12/2/2016

Other Names HBs Ag
How to Order
Check the Hepatitis B Surface Antigen box on the Virology/Immunology Request Form (form 42).
Availability
All clients
Methodology
Enzyme Immunoassay (EIA)
Performed
Daily on regular business days
Time to Reporting
1 to 3 working days after receipt of specimen
CPT Code
87340


Specimen Collection Details

Collection
One 7 ml red top tube or serum separator tube (SST). Also acceptable are specimens collected with anticoagulants EDTA, heparin. Samples collected using anticoagulant should be filled as per manufacturer’s instructions to avoid improper dilution.
Optimal Volume
Blood: 5 ml; Serum 1.5 ml
Minimum Volume
Blood: 3 ml; Serum 0.75 ml
Handling
After blood clots, keep specimen refrigerated until transport. DO NOT FREEZE WHOLE BLOOD. If possible remove the serum from the clot as soon as possible. Specimen must be received within 3 calendar day of specimen collection or sent as frozen serum.  
Transport
Refrigerated at 2-8° C.
Rejection Criteria
Specimens contaminated with bacteria or particulate matter, cadaver specimens, gross lipemia, icteric, and hemolyzed specimens, heat-inactivated specimens, pooled specimens, diluted specimens, specimens in sodium citrate anticoagulant, and specimens received more than 7 days after specimen collection, if they are not frozen.
 
Specimens are subject to the requirements of the OSPHL Specimen Submission Policy and Criteria, available at: http://bit.ly/SpecimenCriteria.
Comments
This test is intended for the qualitative detection of hepatitis B surface antigen in human serum or plasma and also in cadaver specimens. Positive hepatitis B surface antigen tests are repeated in duplicate and low positives are confirmed by neutralization testing.