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Hepatitis B Surface Antigen | | Updated | 10/5/2009 | | Other Names | HBs Ag | | How to Order | Check the Hepatitis B Surface Antigen box on the Virology/Immunology Request Form (form 42) and complete the reason for test under Billing Information Only | | Availability | All clients | | Methodology | Enzyme Immunoassay (EIA) | | Performed | Monday, Wednesday, Friday | |
Time to Reporting
| 1 to 3 working days after receipt of specimen | |
CPT Code
| 87340 |
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Specimen Collection Details
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Collection
| One 7 ml red top tube or serum separator tube (SST). Also acceptable are specimens collected with anticoagulants such as EDTA, citrate, heparin, CPDA-1, and ACD. Samples collected using anticoagulant should be filled as per manufacturer’s instructions to avoid improper dilution. | |
Optimal Volume
| Blood: 5 ml; Serum 1.5 ml | |
Minimum Volume
| Blood: 3 ml; Serum 0.75 ml | |
Handling
| After blood clots, keep specimen refrigerated until transport. DO NOT FREEZE. If possible remove the serum from the clot as soon as possible. Sample must be tested within 7 days of collection. | |
Transport
| Refrigerated at 2-8° C. | |
Rejection Criteria
| Specimens contaminated with bacteria are unacceptable for testing. | |
Comments
| This test is intended for the qualitative detection of hepatitis B surface antigen in human serum or plasma and also in cadaver specimens. Positive hepatitis B surface antigen tests are repeated in duplicate and low positives are confirmed by neutralization testing. |
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