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Hepatitis B Surface Antigen

Updated

10/5/2009

Other Names
HBs Ag
How to Order
Check the Hepatitis B Surface Antigen box on the Virology/Immunology Request Form (form 42) and complete the reason for test under Billing Information Only
Availability All clients
Methodology
Enzyme Immunoassay (EIA)
Performed Monday, Wednesday, Friday
Time to Reporting 1 to 3 working days after receipt of specimen
CPT Code
87340


Specimen Collection Details

Collection
One 7 ml red top tube or serum separator tube (SST). Also acceptable are specimens collected with anticoagulants such as EDTA, citrate, heparin, CPDA-1, and ACD. Samples collected using anticoagulant should be filled as per manufacturer’s instructions to avoid improper dilution.
Optimal Volume Blood: 5 ml; Serum 1.5 ml
Minimum Volume Blood: 3 ml; Serum 0.75 ml
Handling
After blood clots, keep specimen refrigerated until transport.  DO NOT FREEZE.  If possible remove the serum from the clot as soon as possible. Sample must be tested within 7 days of collection.   
Transport
Refrigerated at 2-8° C.
Rejection Criteria Specimens contaminated with bacteria are unacceptable for testing.
Comments
This test is intended for the qualitative detection of hepatitis B surface antigen in human serum or plasma and also in cadaver specimens. Positive hepatitis B surface antigen tests are repeated in duplicate and low positives are confirmed by neutralization testing.