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Influenza Type A and B, Qualitative Real-Time RT-PCR with Reflex

Updated

4/2/2012

Other Names Flue A and B PCR, Influenza PCR
How to Order
Check the 'Other' box under the Virus Isolation heading on the Virology /Immunology Request (form 42) and write in 'RT-PCR for Influenza'
Availability
Patients who fall within the current Acute and Communicable Disease Program Respiratory Surveillance guidelines, Outbreaks, or with ACDP approval
Methodology
Real-Time Reverse Transcriptase Polymerase Chain Reaction (RT-PCR)
Performed
Twice weekly or more, depending on volume
Time to Reporting
2-5 working days after receipt of specimen
CPT Code
87798 x 2. If Influeza A is detected, additionally 87798 x 4 (H1, H3, H1N1 (2009) pdm InfA, and HIN1 (2009) pdm H1)


Specimen Collection Details

Collection
Synthetic nasopharyngeal or nasal swabs in viral transport media. For more information see Instructions for Submitting Influenza Specimens at http://public.health.oregon.gov/LaboratoryServices/SubmittingSamples/Pages/submitting-flu.aspx
Optimal Volume
2-3 mL
Minimum Volume
1 mL
Handling
Keep refrigerated after collection.
Transport
Transport refrigerated at 2-8 degrees C within 5 days.
Rejection Criteria
Specimens contaminated with bacteria or that have been heat-treated are unacceptable for testing.
Comments
This test will identify influenza A H3, seasonal H1, and 2009 H1 subtypes in addition to influenza B.