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Mycobacteriology: M. tuberculosis Complex Susceptibility Testing

Updated

12/13/2009

Other Names
Streptomycin, Isoniazid, Rifampin, Ethambutol, (SIRE), PZA
How to Order
Check the M. tuberculosis Complex Susceptibility Testing  box on the red General Microbiology Request Form
Availability

Primary Samples: County and State Agencies

AFB Isolates: Private Care Laboratories
Methodology

MGIT Method SIRE (preliminary)  and MGIT PZA

Indirect Plate Method: includes SIRE (Final) also Ethionamide, Kanamycin, PAS. 

Ofloxacin is available on PH approval
Performed
Daily
Time to Reporting

MGIT Method: Approximately 2 weeks from ID of growth.

Indirect Plate Method: Approximately 4 weeks.
CPT Code
No charge


Specimen Collection Details

Collection

Primary respiratory samples from County Health Departments are collected as per instructions at:

http://public.health.oregon.gov/LaboratoryServices/Documents/tb.pdf

Susceptibility testing is done on the first isolate on new cases of TB. 

 

TB isolate growth, on new cases, from Private Care Laboratories: submitted as per instructions at: http://public.health.oregon.gov/LaboratoryServices/Documents/tb.pdf  Pages 3-4
Optimal Volume

Primary County respiratory samples:  1 – 5 ml

Private Care Lab AFB Isolates: Viable, Pure growth in/on media.
Minimum Volume

Primary County respiratory samples:  1 ml

Private Care Lab AFB Isolates: Viable, Pure growth in/on media. 

Handling
Room Temperature
Transport

Primary County samples: Delivery should be <5 days after collection

Use appropriate shipping requirements.
Rejection Criteria
Unlabeled specimens, specimens not submitted per collection instructions, or without completed requisition forms will not be processed.
Comments
TB isolates that grow on case samples more than 3 months after the first tested sample will have repeat Susceptiblity testing performed.