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Mycobacterium - M. tuberculosis Complex Susceptibility Testing

Updated

12/2/2016

Other Names Mycobacterium tuberculosis
How to Order
Check the M. tuberculosis Complex Susceptibility Testing  box on the red General Microbiology Request Form
Availability

AFB Isolates: Private Care Laboratories

Methodology

MGIT Method SIRE (preliminary) and MGIT PZA

Indirect Plate Method: includes SIRE (Final) also Ethionamide, Kanamycin, PAS, Ofloxacin.
Performed
Daily on regular business days.
Time to Reporting

MGIT Method: Approximately 2 weeks from receipt of specimen.

Indirect Plate Method: Approximately 4 weeks.
CPT Code
 
 


Specimen Collection Details

Collection

Submit TB culture isolates on Lowenstein-Jensen, 7H10, or other comparable solid AFB media. Failure to submit pure cultures may delay or adversely affect the identification process.

TB "Positive" liquid media tubes from automated TB systems, such as MGIT or BTA, will also be accepted for testing.
 
Submitters should retain subcultures of all submissions as a backup precaution.
Optimal Volume

Viable, Pure growth in/on media.

Minimum Volume

Viable, Pure growth in/on media.

Handling
Store isolate at room temperatures pending transport.
Transport

Transport specimens at room temperatures.

Rejection Criteria
Specimens are subject to the requirements of the OSPHL Specimen Submission Policy and Criteria, available at: http://bit.ly/SpecimenCriteria.
Comments
Submitters should retain subculturesof all submissions as a backup precaution.
 
TB isolates that grow on case samples more than 3 months after the first tested sample will have repeat Susceptiblity testing performed.
 
For additional information or assistance, contact the laboratory at 503-693-4100.