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Brucella Antibody, Total

Updated

9/22/2009

Other Names
How to Order
Check the Brucellosis box on the Virology/Immunology Request (Form 42)
Availability
All clients
Methodology
Bacterial Agglutination
Performed
Weekly
Time to Reporting
1 to 6 working days after receipt of specimen
CPT Code
86622


Specimen Collection Details

Collection
One 7 mL red top or serum separator (SST) tube
Optimal Volume
Blood: 5-7 mL; Serum 1-2 mL
Minimum Volume
Blood: 3 mL; Serum 1 mL
Handling
After blood clots, keep specimen refrigerated until transport. DO NOT FREEZE. If possible remove the serum from the clot as soon as possible. Sample must be tested within 7 days of collection.
Transport
Refrigerated at 2-8° C
Rejection Criteria
Severely lipemic or grossly hemolyzed specimens; specimens received without proper patient identifiers on specimen tube and/or request form; and specimens received with requests without submitter information will be rejected.
Comments
Cross-reactions may occur between Brucella and F. tularensis antigens and antisera; therefore, these antigens should be run in parallel when testing. A fourfold rise in titer ÿ 1:160 is considered diagnostic. A single serum titer of 1:160 or 1:320 is suggestive of brucellosis when accompanied by a compatible clinical course in a patient with a history of potential exposures. Parallel testing of acute and convalescent specimens is preferred.