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Brucella Antibody, Total | | Updated | 9/22/2009 | | Other Names | Undulant Fever | | How to Order | Check the Brucellosis box on the Virology/Immunology Request (Form 42) | | Availability | All Clients | | Methodology | Bacterial Agglutination | | Performed | Weekly | |
Time to Reporting
| 1 to 6 working days after receipt of specimen | |
CPT Code
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Specimen Collection Details
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Collection
| One 7 mL red top or serum separator (SST) tube | |
Optimal Volume
| Blood: 5-7 mL; Serum 1-2 mL | |
Minimum Volume
| Blood: 3 mL; Serum 1 mL | |
Handling
| After blood clots, keep specimen refrigerated until transport. DO NOT FREEZE. If possible remove the serum from the clot as soon as possible. Sample must be tested within 7 days of collection. | |
Transport
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Rejection Criteria
| Severely lipemic or grossly hemolyzed specimens; specimens received without proper patient identifiers on specimen tube and/or request form; and specimens received with requests without submitter information will be rejected. | |
Comments
| Cross-reactions may occur between Brucella and F. tularensis antigens and antisera; therefore, these antigens should be run in parallel when testing. A fourfold rise in titer ÿ 1:160 is considered diagnostic. A single serum titer of 1:160 or 1:320 is suggestive of brucellosis when accompanied by a compatible clinical course in a patient with a history of potential exposures. Parallel testing of acute and convalescent specimens is preferred. |
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