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Middle Eastern Respiratory Syndrome Coronavirus (MERS-CoV), RT-PCR

Updated

12/2/2016

Other Names Novel Coronavirus 2012
How to Order

Check the “Other” box Under the Other heading on the Virology/Immunology Request form (form 42) and write in “MERS-CoV testing”

Availability
With State Acute and Communicable Disease Prevention Program (ACDP) approval only – Healthcare Providers of patients who meet travel requirements for MERS exposure​
Methodology

Real- time reverse transcriptase polymerase chain reaction (rRT-PCR)

Performed

As needed

Time to Reporting

1-3 working days after receipt of specimen.

CPT Code

87798​



Specimen Collection Details

Collection
Refer to Interim Guidelines for Collecting, Handling, and Testing Clinical Specimens from Patients Under Investigation (PUIs) for Middle East Respiratory Syndrome Coronavirus (MERS-CoV) found at:
www.cdc.gov/coronavirus/mers/guidelines-clinical-specimens.html 
Optimal Volume

Refer to Interim Guidelines above

Minimum Volume

1 mL​

Handling

Keep refrigerated after collection. Specimens that are not tested within 72 hours of collection must be frozen at -70ºC and transported on dry ice.

Transport
Transport refrigerated at 2-8ºC for receipt at the OSPHL within 3 calendar days​. If specimen has been frozen, transport using dry ice to maintain frozen temperatures.
Rejection Criteria
For upper and lower respiratory swabs: specimens collected with wooden or calcium alginate swabs.
 
Specimens are subject to the requirements of the OSPHL Specimen Submission Policy and Criteria, available at http://bit.ly/SpecimenCriteria.
Comments

Testing for MERS-CoV should not be performed unless the patient meets the clinical and epidemiological criteria for testing suspect specimens.