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Middle Eastern Respiratory Syndrome Coronavirus (MERS-CoV), RT-PCR

Updated

7/31/2014

Other Names Novel Coronavirus 2012
How to Order

Check the “Other” box Under the Other heading on the Virology/Immunology Request form (form 42) and write in “MERS-CoV testing”

Availability

With ACDP approval only – Healthcare Providers of patients who meet travel requirements for MERS exposure

Methodology

Real- time reverse transcriptase polymerase chain reaction (rRT-PCR)

Performed

As needed

Time to Reporting

1-3 working days after receipt of specimen.

CPT Code

87798​



Specimen Collection Details

Collection

Nasopharyngeal and/or Oropharyngeal swabs, sputum, lower respiratory tract aspirates/washes, serum, stool. Refer to Interim Guidelines for Collecting, Handling, and Testing Clinical Specimens from Patients Under Investigation (PUIs) for Middle East Respiratory Syndrome Coronavirus (MERS-CoV) found at http://www.cdc.gov/coronavirus/mers/guidelines-clinical-specimens.html   http://www.cdc.gov/coronavirus/mers/laboratory-specimen-guidance.html

Optimal Volume

Refer to Interim Guidelines above

Minimum Volume

1 mL​

Handling

Keep refrigerated after collection. Specimens that are not tested within 72 hours of collection must be frozen at -70ºC and transported on dry ice.

Transport

Transport refrigerated at 2-8ºC within 3 days

Rejection Criteria

Samples collected with wooden or calcium alginate swabs. Specimens that do not meet proper collection, storage, or transport guidelines.

Comments

Testing for MERS-CoV should not be performed unless the patient meets the clinical and epidemiological criteria for testing suspect specimens.