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Hepatitis A Antibody, Total (IgM & IgG)

Updated

10/2/2009

Other Names Anti-HAV Total; HAV Ab Total
How to Order
Check the Hepatitis A Total Antibody box on the Virology/Immunology Request Form (form 42)
Availability
All clients
Methodology
Enzyme Immunoassay (EIA)
Performed
Tuesdays and Thursdays on regular business days
Time to Reporting
1 to 8 working days after receipt of specimen
CPT Code
86708


Specimen Collection Details

Collection
One 7 ml red top tube or serum separator tube (SST). Also acceptable are specimens collected with anticoagulants EDTA and heparin. Sodium citrate is not an acceptable anticoagulant. Follow manufacturer’s instructions carefully when using anticoagulant tube containers.
Optimal Volume
Blood: 5 ml; Serum 1.0 ml
Minimum Volume
Blood: 3 ml; Serum 0.5 ml
Handling
After blood clots, keep specimen refrigerated until transport. DO NOT FREEZE WHOLE BLOOD. If possible remove the serum from the clot as soon as possible. Specimen must be received within 4 calendar day of specimen collection or sent as frozen serum. 
Transport
Refrigerated at 2-8° C.
If frozen, package and ship to keep the specimen frozen (e.g., dry ice)
Rejection Criteria
Specimens contaminated with bacteria or particulate matter, gross lipemia, cadaver specimens, specimens collected using citrate anticoagulants, heat-inactivated specimens, specimens received at room temperature, and specimens received more than 7 days after specimen collection, if they are not frozen.
 
pecimens are subject to the requirements of the OSPHL Specimen Submission Policy and Criteria, available at: http://bit.ly/SpecimenCriteria.
Comments
This assay is indicated for use as an aid in the clinical laboratory diagnosis of current or previous hepatitis A virus infection and as an aid in identification of HAV- susceptible individuals for vaccination. This assay tests for both IgM and IgG but does not differentiate between them. This test is intended for use in the qualitative determination of total antibodies to hepatitis A virus in human serum or plasma.