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Clostridium botulinum Toxin

Updated

8/15/2013

Other Names
Botulism; Food poisoning; C. botulinum; Infant Botulism 
How to Order
Select Botulism Toxin under MISCELLANEOUS  section of General Microbiology Request Form (form 60)
Availability
Local health departments and Oregon Public Health Division Office of Disease Prevention and Epidemiology
Methodology
Standard FDA and Laboratory Response Network (LRN) reference procedures; mouse neutralization assay
Performed
Daily, on regular business days
Time to Reporting
4 to 10 working days
CPT Code
87001 – animal inoculation with observation
87076 – anaerobic culture definitive identification
87999 – mouse neutralization bioassay


Specimen Collection Details

Collection
Collection kits available on request (503-693-4114).
Infant Botulism: stool (approx. 2 oz. raw stool without transport media).
Adult Botulism: stool (approximately 2 oz. raw stool without transport media) and serum (minimum of 5 ml); suspect food item (s).
Wound Botulism: specimen from wound in anaerobe transport media, stool (approximately 2 oz. raw stool without transport media), and serum (minimum of 5 ml).
Optimal Volume
Food; all remaining suspect food package including brand information, lot numbers and expiration date. Stool; (approximately 5 ml/2 oz. without transport media).
Minimum Volume
Food; any remaining suspect food; stool; stool swab specimen.
Handling
Hold serum, stool, or food samples at 2-8C.
Transport
Wet ice.
Rejection Criteria
Leaking containers, unlabeled specimens, specimens submitted with incomplete requisition forms or specimens submitted without state or local health department authorization will not be processed.
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