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Hepatitis B Core Antibody, Total (IgM & IgG)

Updated

10/2/2009

Other Names Anti-HBc, HBc Ab, Hepatitis B Pre-vaccine screen
How to Order
Check the Hepatitis B Core Antibody box on the Virology/Immunology Request Form (form 42) and complete the reason for test under Billing Information Only
Availability
All clients
Methodology
Enzyme Immunoassay (EIA)
Performed
Monday, Wednesday, Friday
Time to Reporting
1 to 3 working days after receipt of specimen
CPT Code
86704


Specimen Collection Details

Collection
One 7 ml red top tube or serum separator tube (SST). Also acceptable are specimens collected with anticoagulants such as EDTA, sodium citrate or heparin. Samples collected using anticoagulant should be filled as per manufacturer’s instructions to avoid improper dilution.
Optimal Volume
Blood: 5 ml; Serum 1.0 ml
Minimum Volume
Blood: 3 ml; Serum 0.5 ml
Handling
After blood clots, keep specimen refrigerated until transport. DO NOT FREEZE. If possible remove the serum from the clot as soon as possible. Sample must be tested within 7 days of collection.   
Transport
Transport refrigerated at 2-8° C.
Rejection Criteria
Specimens contaminated with bacteria or particulate matter, cadaver specimens, lipemic, icteric, and hemolyzed specimens. Heat-inactivated samples should be avoided.
Comments
This test is intended for the qualitative determination of total antibodies to hepatitis B core antigen in human serum or plasma. The detection of total Anti-HBc is indicative of exposure to hepatitis B virus (HBV) infection. Further HBV serological marker testing is required to define the specific disease state. This assay tests for both IgM and IgG antibodies but does not differentiate between them.