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Hepatitis B Core Antibody, IgM

Updated

10/2/2009

Other Names Anti-HBc IgM; HBc Ab IgM
How to Order
Check the Hepatitis B Core IgM Antibody box on the Virology/Immunology Request Form (form 42) and complete the reason for test under Billing Information Only
Availability
All clients
Methodology
Enzyme Immunoassay (EIA)
Performed
Tuesday, Thursday
Time to Reporting
1 to 3 working days after receipt of specimen
CPT Code
86705


Specimen Collection Details

Collection
One 7 ml red top tube or serum separator tube (SST). Also acceptable are specimens collected with anticoagulants such as EDTA, sodium citrate or heparin. Samples collected using anticoagulant should be filled as per manufacturer’s instructions to avoid improper dilution.
Optimal Volume
Blood: 5 ml; Serum 1.0 ml
Minimum Volume
Blood: 3 ml; Serum 0.5 ml
Handling
After blood clots, keep specimen refrigerated until transport. DO NOT FREEZE.  If possible remove the serum from the clot as soon as possible. Sample must be tested within 7 days of collection.   
Transport
Transport refrigerated at 2-8° C.
Rejection Criteria
Specimens contaminated with bacteria or particulate matter and cadaver specimens. The use of heat-inactivated samples should be avoided.
Comments
This test is intended for use in the qualitative determination of IgM antibody to hepatitis B core antigen in human serum or plasma. The presence of Anti-HBc IgM, and total Anti-HBc is indicative of acute infection.  The absence of anti-HBc IgM and presence of total Anti-HBc is indicative of recovery from hepatitis B virus (HBV) infection.