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Hepatitis B Surface Antibody

Updated

4/26/2013

Other Names Anti-HBs, HBs Ab, Hepatitis B Post-vaccine screen
How to Order
Check the Hepatitis B Surface Antibody box on the Virology/Immunology Request Form (form 42) and complete the reason for test under Billing Information Only
Availability
All clients
Methodology
Enzyme Immunoassay (EIA)
Performed
Monday, Wednesday, Friday
Time to Reporting
1 to 3 working days after receipt of specimen
CPT Code
86706


Specimen Collection Details

Collection
One 7 ml red top tube or serum separator tube (SST). Also acceptable are specimens collected with anticoagulants such as EDTA, sodium heparin. Samples collected using anticoagulant should be filled as per manufacturer’s instructions to avoid improper dilution.
Optimal Volume
Blood: 5 ml; Serum 1.0 ml
Minimum Volume
Blood: 3 ml; Serum 0.5 ml
Handling
After blood clots, keep specimen refrigerated until transport. DO NOT FREEZE.  If possible remove the serum from the clot as soon as possible. Sample must be tested within 7 days of collection.   
Transport
Refrigerated at 2-8° C.

 

 

Rejection Criteria
Specimens contaminated with bacteria or particulate matter, cadaver specimens, lipemic, icteric, and grossly hemolyzed specimens. Heat-inactivated samples should be avoided.
Comments
This test is intended for the qualitative detection of antibody to hepatitis B surface antibody in human serum or plasma. The detection of anti-HBs is indicative of laboratory diagnosis for seroconversion from hepatitis B virus infection. The test is also used to assess past exposure to hepatitis B in potential hepatitis B vaccine recipients and to determine the presence of immune response to the vaccine. A level of equal to or greater than 10 mIU/ml is generally considered as a protective level for immunity to hepatitis B virus.