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Enteric Pathogen Culture

Updated

4/8/2010

Other Names
Stool culture; stool screen; enteric culture; Salmonella culture; Shigella culture; Escherichia coli O157 culture; EHEC culture; STEC culture; Campylobacter culture; Yersinia culture; Vibrio culture; Aeromonas culture; Plesiomonas culture
How to Order
Check the appropriate box under the CULTURE section of the General Microbiology Request Form (form 60)
Availability
All clients
Methodology
Standard reference procedures for culture of Salmonella, Shigella, Campylobacter and Shiga toxigenic Escherichia coli; submitted samples can also be tested for Vibrio, Yersinia, Aeromonas, and/or Plesiomonas  upon request; any individual organism cultured upon request
Performed
Daily, on regular business days
Time to Reporting
3 to 8 working days
CPT Code
87045 – aerobic stool culture, with isolation and preliminary examination, Salmonella and Shigella  species

87046 – additional pathogens, isolation and presumptive identification of isolates

87077 – additional methods required for definitive identification, each isolate  



Specimen Collection Details

Collection
Collection kits available on request (503-693-4114).

Stool in Enteric Pathogen Transport media.  Swab in EPT acceptable for infant. Detailed instructions for collection and submission of specimens can be found at
https://public.health.oregon.gov/LaboratoryServices/Documents/enteric.pdf

Optimal Volume
5 gm solid stool or 5 ml liquid stool
Minimum Volume
2 gm solid stool or 5 ml liquid stool
Handling
Place in biohazard bag; keep at refrigerator temperature.  Do not freeze, avoid extreme heat. Submit specimen according to Infectious Substance Shipping Guidelines. 
Transport
Wet ice.
Rejection Criteria
Leaking containers, unlabeled specimens, rectal swabs with no visible fecal material, specimens mixed with urine, or specimens submitted with incomplete requisition forms.
Comments
For addition information or assistance, please contact the OSPHL at 503-693-4100.