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Endocrine Disorders



Other Names Congenital Adrenal Hyperplasia (CAH); Congenital Hypothyroidism (CH); Thyroxine (T4); Thyroid Stimulating Hormone (TSH); Newborn screening 
How to Order
Submit Newborn Screening Dried Blood Spot Specimen Collection Card
Birthing centers, medical practitioners and laboratories responsible for newborn screening specimen collection.
Fluorometric immunoassay for 17-hydroxyprogesterone (17-OHP) for CAH; Thyroxine (T4) and Thyroid Stimulating Hormone (TSH) for CH.
Daily, on regular business days, for all specimens received. Routine TSH run on 10% of specimens with the lowest T4 results only.
Time to Reporting
2 to 10 working days after receipt of specimen card.
CPT Code
83498 – Hydroxyprogesterone, 17-d


84437 – Thyroxine; total, requiring elution (eg, neonatal)

84443 – Thyroid stimulating hormone (TSH)

Specimen Collection Details

Capillary blood obtained from heel lance and applied to newborn screening filter paper circles on the specimen collection card.  Obtain specimen prior to transfusion.  Demographic fields should be filled in completely.  First screening specimen should be collected at 24-48 hours of age or before discharge; second screening specimen should be collected at 10-15 days of age.
Optimal Volume
Four evenly saturated circles.
Minimum Volume
Two evenly saturated circles.
Air-dry specimens at room temperature for 2-4 hours in a horizontal position.  Insert dried specimens into a paper, cardboard or Tyvek envelope (do not use plastic) for shipping.  Specimens collected on weekends or holidays should be stored at room temperature and sent by overnight mail or courier at the earliest opportunity.
All specimens should be sent first class or overnight mail or by courier within 24 hours of collection.
Rejection Criteria
Improperly collected specimens, including: uneven saturation, insufficient blood volume, contamination, specimen received more than 14 days after collection or infant is older than 6 months of age.
For more information visit Oregon's Newborn Screening Practitioner's Manual at