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Bordetella PCR

Updated

12/2/2016

Other Names Pertussis PCR; Whooping cough PCR; Bordetella pertussis; Bordetella parapertussis; Bordetella holmesii
How to Order
Check the Bordetella pertussis/parapertussis culture box under CULTURE on the General Microbiology Request Form (form 60).
 
Fill in the date of "Illness Onset" on the form.
Availability
All clients
Methodology
Multiplex Real-Time PCR
Performed
Daily, on regular business days
Time to Reporting
1 to 3 working days after receipt of specimen
CPT Code
87801 x3


Specimen Collection Details

Collection

Collect as soon as possible after symptoms develop. Specimens may be collected up to three weeks after illness onset, as long as antibiotics have not been started.

Collect nasopharyngeal specimen using a Dacron tip swab on a flexible wire shaft and submit in a dry plastic specimen tube.

Nasal aspirates in a sterile tube or container are accepted but are not preferred.

Collect and submit a second specimen following the Bordetella culture instructions if your clinic or patient is in Multnomah, Washington, or Clackamas Counties.

Instructions for NP specimen collection can be found here: public.health.oregon.gov/LaboratoryServices/CommunicableDiseaseTesting/Documents/np-collection.pdf

Optimal Volume
N/A
Minimum Volume
N/A
 
Handling
Store NP and nasal aspirate specimens at refrigerated temperatures pending transport.
Transport
Transport at refrigerated temperatures. Ideally, transport specimens for receipt at the OSPHL within 24 hours of specimen collection.
Rejection Criteria
Specimens received more than 48 hours after specimen collection, non-nasopharyngeal specimens, or specimens not submitted in appropriate transport tube.
 
Specimens are subject to the requirements of the OSPHL Specimen Submission Policy and Criteria, available at: http://bit.ly/SpecimenCriteria.
Comments
PCR assays are routinely performed for B. pertussis, parapertussis, and holmesii.
 
For addition information or assistance, please contact the OSPHL at 503-693-4100.