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Human Immunodeficiency Virus HIV-1 antigen and HIV-1/HIV-2 antibodies

Updated

7/25/2013

Other Names
HIV Antibodies, HIV screen, HIV Antigen/Antibody Combo test
How to Order
CTS submitters check the “Screen” box under the “Non-Rapid Tests Requested” heading on the CTS HIV Test Request pink form 44
Availability
All Clients
Methodology
Enzyme Immunoassay
Performed
Monday through Friday
Time to Reporting
1 to 3 working days after receipt of specimen
CPT Code
87389


Specimen Collection Details

Collection
One 7 ml red top tube or serum separator tube (SST). Tubes with the additives: EDTA and sodium heparin are also acceptable. Please follow collection instructions to avoid improper dilution of samples when using tubes with additives.
Optimal Volume
Blood: 5-7 mL;
Serum 1-2 mL
Minimum Volume
Blood: 1-2 mL
Serum: 0.5 mL
Handling
Blood: After blood clots, keep specimen refrigerated until transport. Samples that will be tested within 3 days of collection can be stored on the clot. If possible remove the serum from the clot as soon as possible. DO NOT FREEZE. Sample must be tested within 7 days of collection.
Transport
Blood: Refrigerated within 5 days
Rejection Criteria
Serum specimens more than 7 days old if not frozen. Specimens collected with additives other than those listed above.
Comments
The test used will detect HIV-1 p24 antigen, HIV-1 & HIV-2 antibodies. Specimens positive by Enzyme Immunoassay will automatically be reflexed to an HIV differentiation test.