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Rubella Antibody, IgG



Other Names German Measles (Rubella)
How to Order
Check the Rubella IgG box on the green Virology form 42. Add the Time of Collection next to the Date of Collection.
All clients
Enzyme Immunoassay (EIA)
Tuesday and Thursday
Time to Reporting
24 - 72 hours after receipt of specimen at OSPHL
CPT Code

Specimen Collection Details

One 7 ml red top tube or serum separator tube (SST).
Optimal Volume
Blood: 5-7 ml; Serum 1-2 ml
Minimum Volume
Blood: 1 ml; Serum 0.5 ml
Blood: After blood clots, keep specimen refrigerated until transport. Samples that will be delivered within 48 hours of collection can be stored on the clot. If possible remove the serum from the clot as soon as possible.
If the sample will not be delivered to the OSPHL within 48 hours, freeze the serum and ship at -20° C on dry ice.
Transport refrigerated at 2-8° C. Specimens must be tested or frozen within 48 hours from specimen collection.
The OSPHL recommends that specimens only be sent from your facility Monday through Thursday. If the specimen cannot be sent to arrive at the laboratory Monday through Friday to meet the 48 hour requirement, then the serum should be frozen and shipped at -20° C on dry ice. Specimens received on Saturdays will be Unsatisfactory for Testing.
Please monitor holiday communications for specific transport considerations.
Rejection Criteria
Specimens that have undergone multiple freeze/thaw cycles, are Hemolyzed, Icteric, heat inactivated or grossly contaminated, not able to be tested or frozen within 48 hours after sample collection, or received on Saturdays are unacceptable for testing.
The level of 10 IU/mL of rubella antibody is accepted as the cutoff for immunity.

A POSITIVE result corresponds to a level > 10 IU/mL. Specimen is positive for anti-Rubella IgG and the patient is presumed to be immune.

A Negative result corresponds to an antibody level <9 IU/mL. Speciemn is negative for anti-Rubella IgG and the patient is presumed to be non-immune.

An Equivocal result corresponds to an antibody level between > 9 but < 10 IU/mL. The specimen is Equivocal for anti-Rubella IgG antibody and the patient’s immune status cannot be determined. A subsequent sample should be drawn and tested simultaneously with the initial sample. If the subsequent sample is positive, seroconversion has occurred and may be indicative of recent infection. If the subsequent sample also tests as Equivocal then antibody status is undetermined and the sample is deemed equivocal.

This test is used for the qualitative and/or semi-quantitative detection of IgG antibodies to rubella in human serum. The Rubella IgG ELISA test is of value in the determination of rubella immune status. Seroconversion between acute and convalescent sera is considered strong evidence of current or recent infection. Note: the Acute phase serum specimen should be drawn as soon after rash onset as possible, within the first 7 days. The Convalescent phase serum should be drawn 10 days or more after the Acute phase specimen.