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Rubella Antibody, IgG

Updated

12/2/2016

Other Names German Measles (Rubella)
How to Order
Check the Rubella IgG box on the green Virology form 42
Availability
All clients
Methodology
Enzyme Immunoassay (EIA)
Performed
Tuesdays and Fridays on regular business days
Time to Reporting
1-3 working days after receipt of specimen
CPT Code
86762


Specimen Collection Details

Collection
One 7 ml red top tube or serum separator tube (SST).
 
Note: If collecting during the Acute phase, serum specimen should be drawn as soon after rash onset as possible, within the first 7 days. If collecting during the Convalescent phase, serum should be drawn 10 days or more after the Acute phase specimen.
Optimal Volume
Blood: 5-7 ml; Serum 1-2 ml
Minimum Volume
Blood: 1 ml; Serum 0.5 ml
Handling
Blood: After blood clots, keep specimen refrigerated until transport. Samples that will be tested within 3 days of collection can be stored on the clot. If possible remove the serum from the clot as soon as possible. DO NOT FREEZE. Sample must be tested within 7 days of collection.
Transport
Transport refrigerated at 2-8° C. Specimens must be tested or frozen within 48 hours from specimen collection.
 
The OSPHL recommends that specimens only be sent from your facility Monday through Thursday. If the specimen cannot be sent to arrive at the laboratory Monday through Friday to meet the 48 hour requirement, then the serum should be frozen and shipped at -20° C on dry ice. Refrigerated specimens received on Saturdays will be Unsatisfactory for Testing.
 
Please monitor holiday communications for specific transport considerations.
Rejection Criteria
Specimens that have undergone multiple freeze/thaw cycles, are Hemolyzed, Icteric, heat inactivated or grossly contaminated, not able to be tested or frozen within 48 hours after sample collection, or refrigerated or room temperature specimens received on Saturdays are unacceptable for testing.
 
Specimens are subject to the requirements of the OSPHL Specimen Submission Policy and Criteria, available at http://bit.ly/SpecimenCriteria.
Comments
This test is used for the qualitative and/or semi-quantitative detection of IgG antibodies to rubella in human serum. The Rubella IgG ELISA test is of value in the determination of rubella immune status. The level of 10 IU/mL of rubella antibody is accepted as the cutoff for immunity.
 
If an equivocal result is reported a second sample should be drawn. If the second sample is reported as positive, seroconversion has occurred and is indicative of a recent infection. If the second sample is reported as equivocal, the antibody status is undetermined.