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Virus Isolation, Rapid Culture with Reflex

Updated

12/2/2016

Other Names Virus Culture; Cell Culture; Coxsackieviruses; Cytomegalovirus (CMV); Echoviruses; Encephalitis Viruses; Enterovirus Culture; Herpes Culture; Influenza Culture; Varicella Culture; Respiratory Syncytial Virus (RSV); Respiratory Virus Culture
How to Order
Check the “Virus Isolation” box on the Virology/Immunology Request Form (form 42). Complete the Suspected Agent and the Date of Illness Onset.
Availability
All submitters
Methodology
Spin Amplified Cell Culture with Reflex to Direct Fluorescent Antibody (DFA) Stain 
Performed
Daily on regular business days
Time to Reporting
2 - 10 working days after receipt of specimen
CPT Code
87254. 87140 x number of additional stains required to identify a positive.


Specimen Collection Details

Collection
Specimen
Common Viruses Isolated
CSF & CNS Tissues
CMV, HSV, enteroviruses, VZV
Dermal Lesions
Adenovirus, enteroviruses, HSV, VZV
Eye
CMV, Adenovirus, enteroviruses, HSV, VZV
Genital 
HSV
Mucosal 
HSV, VZV
Oral 
HSV, VZV
Rectal 
Enteroviruses, HSV, VZV
Respiratory Tract 
 
Upper 
Adenovirus, enteroviruses, HSV, hMPV, influenza, parainfluenza, rhinovirus, RSV
Lower 
Adenovirus, CMV, human metapneumovirus (hMPV), influenza, parainfluenza, RSV
Stool 
Adenovirus, enteroviruses
Tissues 
CMV, enteroviruses, HSV
Urine 
Adenovirus, CMV, enteroviruses

Specimen submission criteria for each specimen source can be found here: public.health.oregon.gov/LaboratoryServices/CommunicableDiseaseTesting/Documents/virusisolation.pdf

Optimal Volume
Specimen volume dependent upon specimen source. See the OSPHL instructions here: public.health.oregon.gov/LaboratoryServices/CommunicableDiseaseTesting/Documents/virusisolation.pdf

Minimum Volume
Specimen volume dependent upon specimen source. See the OSPHL instructions here: public.health.oregon.gov/LaboratoryServices/CommunicableDiseaseTesting/Documents/virusisolation.pdf
Handling
Specimen submission criteria for each specimen source can be found here: public.health.oregon.gov/LaboratoryServices/CommunicableDiseaseTesting/Documents/virusisolation.pdf
Transport
Specimen transport requirements dependent upon specimen source. See the OSPHL instructions here: public.health.oregon.gov/LaboratoryServices/CommunicableDiseaseTesting/Documents/virusisolation.pdf
Rejection Criteria
Specimen acceptance criteria may be dependent upon specimen source. Please see the OSPHL instructions here: public.health.oregon.gov/LaboratoryServices/CommunicableDiseaseTesting/Documents/virusisolation.pdf
 
Specimens are subject to the requirements of the OSPHL Specimen Submission Policy and Criteria, available at http://bit.ly/SpecimenCriteria.
Comments

Specimens should be collected as early in the acute stage of infection as possible. Providing date of onset and a brief clinical description (e.g. if patient has rash or respiratory symptoms) can be helpful for virus culture.

It is not practical to try to culture many common viruses that infect humans. Some examples are: hepatitis viruses, arboviruses, papillomaviruses, parvoviruses, reoviruses, and gastrointestinal viruses such as astro, corona, calici, and rotaviruses.

Respiratory cultures: Nasopharyngeal aspirates/washes or swabs are the preferred specimen types. Throat swabs, nasal aspirates, and bronchial washes may also be submitted.

Enterovirus cultures: Rectal swabs, stool, or Nasopharyngeal or lower respiratory tract specimens are acceptable. CSF, eye lesions, tissues, and urines will also be accepted.

For other specimen types or suspected organisms, please consult with OSPHL before submission.