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Clostridium botulinum Toxin

Updated

12/2/2016

Other Names Botulism; Food poisoning; C. botulinum; Infant Botulism 
How to Order
Select the C. Botulinum toxin box under MISCELLANEOUS section of the General Microbiology Request Form (form 60).
 
Notify the OSPHL General Microbiology Section before sending at 503-693-4100.
Availability
All clients with approval from the Oregon Public Health Division Acute and Communicable Disease Prevention Program.
Methodology
Standard FDA and Laboratory Response Network (LRN) reference procedures; mouse neutralization assay
Performed
Daily, on regular business days
Time to Reporting
4 to 14 working days after receipt of specimen
CPT Code
87001 – animal inoculation with observation
87999 – mouse neutralization bioassay


Specimen Collection Details

Collection
Infant Botulism: Submit patient stool in a sterile screw top container.
 
Adult Botulism: Serum is preferred. Serum may be submitted as an aliquot, in a serum seperator tube, or other applicable serum container. Submit patient stool in a sterile screw top container. Submit suspect food item(s), if available. Collect food items aseptically into a sterile container or submit in original container.
 
Wound Botulism: Specimen from wound in anaerobe transport media, stool in a sterile screw top container, and serum as an aliquot, in a serum seperator tube, or other applicable serum container.
Optimal Volume
Stool: 5ml/2oz
Serum: 5mL
Food: 100-200g; If reasonable, submit all remaining suspect food package including brand information, lot numbers and expiration date.
Minimum Volume
 
If optimal volumes cannot be collected, contact the OSPHL General Microbiology staff for specimen collection instructions.
Handling
Hold serum, stool, or food samples at refrigerated temperatures.
Storage at room temperature is acceptable, but not preferred.
Transport
Transport at refrigerated temperatures.
Transport at room temperature is acceptable, but not preferred.
Rejection Criteria
Specimens submitted without state or local health department authorization.
 
Specimens are subject to the requirements of the OSPHL Specimen Submission Policy and Criteria, available at http://bit.ly/SpecimenCriteria.
Comments
​For additional information or assistance, please contact the OSPHL at 503-693-4100.