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Hepatitis B Core Antibody, Total (IgM & IgG)

Updated

12/2/2016

Other Names Anti-HBc; HBc Ab; Hepatitis B Pre-vaccine screen
How to Order
Check the Hepatitis B Core Antibody box on the Virology/Immunology Request Form (form 42)
Availability
All clients
Methodology
Enzyme Immunoassay (EIA)
Performed
Daily on regular business days
Time to Reporting
1 to 3 working days after receipt of specimen
CPT Code
86704


Specimen Collection Details

Collection
One 7 ml red top tube or serum separator tube (SST). Also acceptable are specimens collected with anticoagulants EDTA and heparin. Follow manufacturer’s instructions carefully when using anticoagulant tube containers.
Optimal Volume
Blood: 5 ml; Serum 1.0 ml
Minimum Volume
Blood: 3 ml; Serum 0.5 ml
Handling
After blood clots, keep specimen refrigerated until transport. DO NOT FREEZE WHOLE BLOOD. If possible remove the serum from the clot as soon as possible. Specimen must be received within 4 calendar day of specimen collection or sent as frozen serum.  
Transport
Refrigerated at 2-8° C.
If frozen, package and ship to keep the specimen frozen (e.g., dry ice)
Rejection Criteria
Specimens contaminated with bacteria or particulate matter, cadaver specimens, lipemic, icteric, and hemolyzed specimens, heat-inactivated specimens, pooled specimens, diluted specimens, specimens in sodium citrate anticoagulant, and specimens received more than 7 days after specimen collection, if they are not frozen.
 
Specimens are subject to the requirements of the OSPHL Specimen Submission Policy and Criteria, available at: http://bit.ly/SpecimenCriteria.
Comments
Specimens contaminated with bacteria or particulate matter, cadaver specimens, lipemic, icteric, and hemolyzed specimens, heat-inactivated specimens, pooled specimens, diluted specimens, specimens in sodium citrate anticoagulant, and specimens received more than 7 days after specimen collection, if they are not frozen.
Specimens are subject to the requirements of the OSPHL Specimen Submission Policy and Criteria, available at: http://bit.ly/SpecimenCriteria.